Search results for " gag"
showing 10 items of 49 documents
ESCRT Requirements for Murine Leukemia Virus Release
2016
The Murine Leukemia Virus (MLV) is a gammaretrovirus that hijack host components of the endosomal sorting complex required for transport (ESCRT) for budding. To determine the minimal requirements for ESCRT factors in MLV viral and viral-like particles (VLP) release, an siRNA knockdown screen of ESCRT(-associated) proteins was performed in MLV-producing human cells. We found that MLV VLPs and virions primarily engage the ESCRT-I factor Tsg101 and marginally the ESCRT-associated adaptors Nedd4-1 and Alix to enter the ESCRT pathway. Conversely, the inactivation of ESCRT-II had no impact on VLP and virion egress. By analyzing the effects of individual ESCRT-III knockdowns, VLP and virion releas…
L’abside in facciata: soluzioni “antisismiche” del XVIII secolo in Sicilia
2015
Nel corso del Settecento vennero progettate e costruite in Sicilia facciate chiesastiche caratterizzate da conformazioni curvilinee. Forme convesse e concave, posizionate in corrispondenza del partito centrale della facciata rendono queste strutture assimilabili alle absidi. Scelte di natura estetica hanno inizialmente innescato fenomeni emulativi sulla base della ricezione dei modelli diffusi dalla seconda metà del Seicento in Sicilia dalle incisioni riproducenti le facciate inflesse prodotte dai maestri del Barocco romano. Finora l'interesse verso questi modelli è apparso lo schema interpretativo più pertinente per spiegare gli indirizzi progettuali dei maggiori artefici operanti nel Sett…
María Magdalena. Continuidad y variación en la cultura audiovisual (1897-2014)
2017
El objetivo principal de esta tesis consiste en analizar la presencia de María Magdalena en las producciones audiovisuales del periodo comprendido entre 1897 y 2014. Dichas producciones proceden de Europa, Estados Unidos, Canadá y México. El marco metodológico general a partir del que se plantea el análisis es la iconología, entendida como Zwichenraum o tierra de confluencias. En concreto, la noción de continuidad y variación, iniciada por Aby Warburg y continuada por Fritz Saxl, es la principal cuestión teórica en la que se basa la aproximación. El estudio se lleva a cabo en perspectiva diacrónica con el objetivo de detectar dichas fases de continuidad y variación del personaje a lo largo …
Trattamento laparoscopico del Mielolipoma surrenalico: caso clinico e revisione della letteratura.
2006
Adrenal myelolipoma is a rare benign, non-functioning tumor consisting of fat and hematopoietic tissues. In January 2005 we had observed an adrenal myelolipoma in 70 year old man. During the follow-up for bladder urothelioma, an abdominal CT revaled a well delineated 4x4 cm homogeneous fatty mass in the right suprarenal area with negative attenuation values. The functional study of adrenal gland was normal. The patient underwent videolaparoscopic right adrenalectomy (Gagner technique). Postoperative course was uneventful. The istological diagnosis showed adrenal myelolipoma. We conclude that videolaparoscopic adrenalectomy should be considered the gold standard treatment for benign adrenal …
Free and antibody-complexed antigen and antibody profile in apparently healthy HIV seropositive individuals and in AIDS patients.
1990
The pattern of free and antibody-complexed HIV antigen and the antibody profile were investigated retrospectively in 305 serum samples taken from 22 AIDS patients before and during the development of AIDS and from 40 apparently healthy seropositive individuals. Most AIDS patients were found positive for both free and complexed antigen and had high gp41 antibody titres but low or undetectable p24 antibody. Four different patterns of HIV antigenaemia were observed: 1) positive for both free and complexed antigen; 2) negative for free HIV antigen at first, but always positive for complexed antigen; 3) positive for free antigen without complexed antigen; and 4) negative for both free and comple…
Genetic Diversity of HIV-1 Non-B Strains in Sicily: Evidence of Intersubtype Recombinants by Sequence Analysis ofgag,pol, andenvGenes
2007
The molecular epidemiology of HIV-1 strains in Sicily (Italy) was phylogenetically investigated by the analysis of HIV-1 gag, pol, and env gene sequences from 11 HIV-1 non-B strains from 408 HIV-1-seropositive patients observed from September 2001 to August 2006. Sequences suggestive of recombination were further investigated by bootscanning analysis of various fragments. Overall, we identified several second-generation recombinant (SGRs) strains, which contained genetic material of CRF02_AG in at least one gene. Notably, three individuals were found to be infected with subsubtype A3, and one of them showed genetic recombination with subsubtype A4. The current study emphasizes the genetic a…
Analisi e rappresentazione tridimensionale di due disegni di altari di Rosario Gagliardi
2019
I Barresi di Pietraperzia. Una corte feudale tra medioevo ed età moderna
2016
La ricerca si incentra sulla committenza artistica e in particolare sulle iniziative architettoniche promosse da una famiglia dell'alta aristocrazia feudale in Sicilia, i Barresi di Piatraperzia, un centro ubicato nell’entroterra dell’isola, attualmente in provincia di Enna. In questo luogo, dove i Barresi risiederono stabilmente, si sviluppò tra il XV e il XVI secolo una corte aristocratica colta e sofisticata, che ebbe il suo fulcro nel castello di Pietraperzia, soprattutto grazie a due membri di questa famiglia, Giovanni Antonio II e il figlio Matteo, che intrattennero rapporti con autorevoli esponenti della cultura scientifica, artistica e letteraria del tempo. Il ruolo di mecenati e fi…
Caution. Conditions de la décharge d'une caution. Fait du créancier susceptible de rendre la subrogation impossible. Le non-exercice par le créancier…
2014
International audience; (Com., 9 juillet 2013, n° 12-19.949, F-D, M. X. c / BNP Paribas, Gaz. Pal. 29 sept.-1er oct. 2013, p. 36, note E. Le Corre-Broly)
Structure of SNX9 SH3 in complex with a viral ligand reveals the molecular basis of its unique specificity for alanine-containing class I SH3 motifs
2021
Class I SH3 domain-binding motifs generally comply with the consensus sequence [R/K]x0PxxP, the hydrophobic residue 0 being proline or leucine. We have studied the unusual 0 = Ala-specificity of SNX9 SH3 by determining its complex structure with a peptide present in eastern equine encephalitis virus (EEEV) nsP3. The structure revealed the length and composition of the n-Src loop as important factors determining specificity. We also compared the affinities of EEEV nsP3 peptide, its mutants, and cellular ligands to SNX9 SH3. These data suggest that nsP3 has evolved to minimize reduction of conformational entropy upon binding, hence acquiring stronger affinity, enabling takeover of SNX9. The R…